Virgin queen bees have not yet fully developed their reproductive system and therefore are somewhat smaller than mated queen bees.
SIGNS YOUR COLONY IS QUEENLESS - Beekeeping Like A Girl
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Black Bee Honey - A virgin queen went out on her mating flight and returned home only to not go through the front door but take up residence under the hive. A
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BreedingBEST Protocol
Queenlessness - Wildflower Meadows
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The Remarkable World of Native Bees and Their Ecological Impact - WellBeing Magazine